Emerging Family of Proline-Specific Peptidases of Porphyromonas gingivalis : Purification and Characterization of Serine Dipeptidyl Peptidase, a Structural and Functional Homologue of Mammalian Prolyl Dipeptidyl Peptidase IV
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چکیده
منابع مشابه
Emerging family of proline-specific peptidases of Porphyromonas gingivalis: purification and characterization of serine dipeptidyl peptidase, a structural and functional homologue of mammalian prolyl dipeptidyl peptidase IV.
Porphyromonas gingivalis is an asaccharolytic and anaerobic bacterium that possesses a complex proteolytic system which is essential for its growth and evasion of host defense mechanisms. In this report, we show the purification and characterization of prolyl dipeptidyl peptidase IV (DPPIV) produced by this organism. The enzyme was purified to homogeneity, and its enzymatic activity and biochem...
متن کاملExpression, purification and preliminary crystallographic analysis of dipeptidyl peptidase IV from Porphyromonas gingivalis.
The asaccharolytic periodontopathogen Porphyromonas gingivalis produces membrane-anchored proteases such as dipeptidyl peptidase IV that are involved in the destruction of host periodontal tissue. The extracellular domain of this enzyme was overexpressed in Escherichia coli as an N-terminal His-tag fusion protein, purified using standard metal-affinity chromatography and crystallized using the ...
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We found that N-unblocked nine p-nitroanilde derivatives of amino acids or peptides were hydrolyzed by the crude cell extracts of Streptococcus anginosus NCTC 10713. Then dipeptidyl peptidase IV was purified 323-fold by the procedures including ammonium sulfate concentration, anion exchange chromatography (twice), gel filtration (twice), hydrophobic interaction chromatography, and isoelectric f...
متن کاملCrystal structure analyses of dipeptidyl peptidase 11 from Porphyromonas gingivalis
Yasumitsu Sakamoto1, Saori Roppongi1, Ippei Iizuka1, Yoshio Kusakabe2,3, Tomonobu Umeda2,4, Yuki Odanaka2, Satoko Matsubayashi2, Yasuyuki Kitagawa2,4, Takamasa Nonaka1 and Nobutada Tanaka2,* 1School of Pharmacy, Iwate Medical University, 2-1-1 Nishitokuta, Yahaba, Iwate 028-3694, Japan 2School of Pharmacy, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan 3Faculty of Pharma...
متن کاملDipeptidyl peptidases 8 and 9: specificity and molecular characterization compared with dipeptidyl peptidase IV.
Dipeptidyl peptidases 8 and 9 have been identified as gene members of the S9b family of dipeptidyl peptidases. In the present paper, we report the characterization of recombinant dipeptidyl peptidases 8 and 9 using the baculovirus expression system. We have found that only the full-length variants of the two proteins can be expressed as active peptidases, which are 882 and 892 amino acids in le...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 2000
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.68.3.1176-1182.2000